After completion of the course, the student:
1. knows mathematical concepts used in NMR, in particular;
· use of spin operators, spin Hamiltonian, matrix representation of spin operators
· quantum mechanical description of a J-coupled spin system (weak and strong coupling)
· calculating a NMR spectrum (energy levels, transition probability)
2. knows modern methods to describe NMR experiments, in particular;
· product operator formalism, shape/meaning of product operators, coherence transfer
· time evolution of product operators (effect of pulses, evolution under the spin hamiltionian)
· description of NMR pulse sequences using product operators
· multiple quantum coherence
3. knows basic NMR experiments and building blocks (using product operators), in particular;
· spin-echo experiments (with and without J-coupling)
· INEPT
· homonuclear 2D NMR (COSY, double-quantum filtered COSY, NOESY, TOCSY)
· heteronuclear 2D NMR (HSQC, HMQC)
· basic 3D NMR experiments (NOESY-HSQC, HNCO)
4. is acquainted with advanced topics in modern biomolecular NMR literature, in particular
· methods (pulsed field gradients, TROSY)
· interactions (residual dipolar coupling)
· relaxation theory (modelfree analysis, cross-correlated relaxation)
· paramagnetic NMR (pseudo-contact shifts, paramagnetic relaxation enhancement)
|