After this course you will be able to:

1.    understand and implement a published bio-analysis method;

2.    set up a method for the analysis of a drug in a biological matrix;

3.    describe the power and problems of LC-MS;

4.    process and review experimental bio-analysis data;

5.    write and present experimental data in a precise and concise way.

 

Contents
In this course you will become acquainted with the analysis of drugs, metabolites and endogenous substances in biological matrices (blood, urine, etc.).The detection of low concentrations of a compound in a complex matrix is often needed, but also enormously challenging. During this course attention will be paid to the design of bio- analysis methods, the problems encountered and possible solutions.

The course is divided into 3 parts: sample preparation, separation and detection, and HPLC in combination with mass spectrometry (LC-MS). These subjects will be explored based on a case/problem in small groups using different techniques. The theory is mastered by reading literature and presenting the main points in lectures to each other. The experiments are completely designed and conducted by you, with input by supervisors, mainly during presentations of your plans en results.

Assessment
The grade is based on the experimental work skills, on the contribution to the discussions, and the quality of the research report and the research presentations.