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Working methods
In this course the project gaining theoretical and practical knowledge on Analytical methods forms the central part. It will consist of literature search and real experiments in the laboratory. In addition, there will be “Meet-the Expert” lectures, assignments, practical work in the laboratory, computer simulation, oral presentations and report audits. We will further have site visits to the Flow cytometry core facility of the UU, and Pamgene.
Course Aims
1. Gain basic knowledge on chemical and biological analytical techniques (spectroscopy, liquid chromatography, NMR, mass spectrometry, PCR, protein analysis, immunodetection, flow cytometry)
Through self study of text books and internet sources basic theoretical knowledge on diverse analytical techniques is created. A number of techniques will be made available to “solve” the CSI cases and students will gain practical experience with these techniques.
2. How to setup an analytical experiment.
The student can formulate the problem/question at hand, find the corresponding literature and come with an experimental design that is feasible and appropriate for the question/problem.
Key words: estimation of the problem, experimental design, applicability
3. Performing an chemical/biological analytical experiment
The student can set up and perform the designed experiment conform the analytical standards. The student performs the experiment such that the results obtained are accurate and reproducible.
Key words: lab safety, lab journal, chemical calculations
4. Data analysis and statistics
The student can analyze the obtained data, perform the essential statistics and interpret the results.
Key words: accuracy, precision, reproducibility
Flanking’ – Education
- Lectures
- Assignments
- Meet the experts
- Internal tutorials on techniques available
- Site visits
Assessment:
1. Individual exam on theory Analytical Methods (week 5)
2. Practical work, lab notebook
3. Final presentations
Coördinators
Dr. M. Alterlaar,Division of Biomolecular Mass Spectrometry and Proteomics, room Kruyt O605, tel: 030-2539554, e-mail: m.altelaar@uu.nl
Dr. F.A.M. Redegeld, Division of Pharmacology, DDW2.88 , tel: 06-20252139, e-mail: f.a.m.redegeld@uu.nl.
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