The student knows:
•the most important physical aspects of light microscopy
•the relative importance of the various factors
•the most important principles of special microscopic techniques as phase contrast and differential interference contrast
•the most important physical aspects of fluorescence
•the most important physical aspects of fluorescence microscopy: single and two-photon excitation microscopy
•the possibilities of fluorescence lifetime microscopy
•the possibilities and limitations of all these microscopy techniques
•the relationship between microscopic imaging and electronically acquired images
•the possibilities and dangers of application of these techniques to living tissue
The student can:
•work with a standard microscope
•describe and select in a correct way the most important properties of the various microscope types
•advise of the choice of various microscope parts on the basis of product information
•advise of the coherence of the various components of a microscope
•indicate what type of microscope is required for a certain research question
•understand and interpret literature in the field of microscopy
•advise of the use of various types of microscopes for living tissues.
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Period (from – till): Quartile 4, timeslot B1 (B1 - ma 5-6, wo 1-2), 20 April - 3 July 2020
Faculty
Responsible lecturer: dr.ir. M.C. van Turnhout - GEM-Z 4.107 - 2797
Co-lecturer: dr.ir. J.P.M. Hoefnagels
Information:Secr. Soft Tissue Biomechanics and Engineering - GEM-Z 4.115 - 2279
Description of content
PLEASE NOTE THAT THIS COURSE IS TAUGHT AT TU/e
The course is meant for students that may have to do light microscopy on biological samples for their internship(s) or MSc-project, and for students that are interested in biomedical applications of optics in general. After completion of this course one has obtained a thorough background in the possibilities and limitations of various optical-microscopic techniques, in particular the possibilities and limitations related to biological samples.
Literature/study material used:
Handouts of lectures, Extra information handed out during the lectures. Students are expected to find their own way in literature (recommended).
Douglas B. Murphy & Michael W. Davidson; Fundamentals of light microscopy and electronic imaging (2nd edition); Wiley-Blackwell, October 2012; ISBN 978-0-471-69214-0 (recommended)
Registration:
Please register at the TU/e, course code: 8MM10. Osiris registration will be done retroactively when results from the TU/e are received.
Mandatory for students in own Master’s programme:
No
Optional for students in other GSLS Master’s programme:
No, for RMTM students only.
Prerequisite knowledge:
8NC00 - Electromagnetism and optics (recommended)
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